Composite

Part:BBa_K5048013:Design

Designed by: Yecheng Zhao   Group: iGEM24_WHU-China   (2024-09-13)


T7-lac-Lpp'OmpA(codon optimised)-DDDDK-QEP-6xhis-dt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 426
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We add enterokinase site between Lpp'OmpA and small peptides in order to verify the enzymatic cleavage.


Source

complete synthesis

References